Bacterial Community Structure in Sago Pith and Sago Waste Water and Its Potential Uses as Organic Acids Producer

Sago is one of commodities in South Sulawesi. The extraction process sago flour produces waste water that still contains organic matter and smells sour. So, it has the potential as a pollutant. sour odor produced allows presence acid-producing bacteria can be used for treatment. objective this research to explore bacterial community structure pith through high-throughput sequencing technology its uses acids producer. Samples were obtained from traditional factory Palopo City, Sulawesi, Indonesia. acidity degree, total dissolve solid, temperature measured pool at sampling area, while nutrient contents analyzed by titration method Testing Laboratory Food Quality Safety, Brawijaya University. Bacterial cells acquired multilevel filtering on filter paper with pore nitrocellulose membrane sizes 0.45 0.20 µm. Total DNA trapped size µm was isolated using FastDNA Spin Kit (MPBIO) V3-V4 regions 16S rDNA (341f-806r) amplified. Amplicons Miseq Illumina further Muscle v.3.8.31, QIIME v.1.7.0, R v.2.15.3. result shows content higher than water. Both samples are dominated Phylum Proteobacteria share 189 common species. dominant produce acid Dysgonomonas sp., Propionispira Lactobacillus pentosus. While, mali Gluconobacter frateurii acid-producer Keywords: structure, Next generating sequencing, pith, wastewater